Control:Article Title: HSV-1 and Zika Virus but Not SARS-CoV-2 Replicate in the Human Cornea and Are Restricted by Corneal Type III Interferon
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human interferon lambda receptor 1 neutralizing mAb PBL assay science Cat# 21885-1 Unable to locate RRID Mouse IgG1 kappa isotype control mAb Sigma-Aldrich Cat# M7894, RRID:AB_1163632 Bacterial and Virus Strains Herpes simplex virus-1 strain 17+ Bertke et al., 2013 N/A Zika virus, strain Paraiba 2015 (Brazil, 2015) Magnani et al., 2018 N/A SARS-CoV-2 (strain 2019 n-CoV/USA_WA1/2020) CDC/BEI Resources NR52281 Biological Samples Human corneas Mid-America Transplant Institute N/A Chemicals, Peptides, and Recombinant Proteins Recombinant Human IL-29/IFN Lambda 1, HCE PBL assay science Cat# 11725-1 Recombinant Mouse IL-28B/IFN Lambda 3 PBL assay science Cat# 12820-1 Critical Commercial Assays RNeasy Mini Kit QIAGEN Cat# 74104 TaqMan RNA-to-Ct 1-Step Kit ThermoFisher Cat# 4392653 Experimental Models: Cell Lines Vero CCL81 cells ATCC CCL81; RRID: CVCL_0059 Vero E6 cells ATCC CRL-1586; RRID: CVCL_0574 Experimental Models: Organisms/Strains Mouse: Ifnar1 / Jackson Laboratory Cat# JAX:032048, RRID: IMSR_JAX:032048 Mouse: AG129 Marshall BioResources AG129 Mouse: Ifnlr1 / Baldridge et al., 2017 N/A Oligonucleotides SARS-CoV-2 N F: 50 -ATGCTGCAATCGTGCTACAA-3 Hassan et al., 2020 N/A SARS-CoV-2 N R: 50 -GACTGCCGCCTCTGCTC-30 Hassan et al., 2020 N/A SARS-CoV-2 N Probe: 50 -/56-FAM/TCAAGGAAC/ZEN/ AACATTGCCAA/3IABkFQ/-30 Hassan et al., 2020 N/A RNAscope Probe- V-ZIKV Advanced Cell Diagnostics Cat# 467771 RNAscope Probe- Hs-IFNLR1 Advanced Cell Diagnostics Cat# 494771 RNAscope Probe- Hs-IFNL1 Advanced Cell Diagnostics Cat# 412341 IFNL1 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.56a.21113836.g IFNL2 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.56a.38564463 IFNLR1 PrimeTime qPCR Assay Integrated DNA Technologies Hs00417120_m1 IFIT1 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.56a.20769090.g IFIT2 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.58.1099131 IRF7 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.58.24613215.g IFI27 PrimeTime qPCR Assay ThermoFisher Hs01086373_g1 GAPDH PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.39a.22214936 Ifit1 PrimeTime qPCR Assay Integrated DNA Technologies Mm.PT.58.32674307 Ifit2 PrimeTime qPCR Assay Integrated DNA Technologies Mm.PT.58.45910930.g Ifitm3 PrimeTime qPCR Assay Integrated DNA Technologies Mm.PT.56a.29591636 Irf7 PrimeTime qPCR Assay ThermoFisher Mm00516793 (Continued on next page) e1 Cell Reports 33, 108339, November 3, 2020
Virus:Article Title: HSV-1 and Zika Virus but Not SARS-CoV-2 Replicate in the Human Cornea and Are Restricted by Corneal Type III Interferon
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human interferon lambda receptor 1 neutralizing mAb PBL assay science Cat# 21885-1 Unable to locate RRID Mouse IgG1 kappa isotype control mAb Sigma-Aldrich Cat# M7894, RRID:AB_1163632 Bacterial and Virus Strains Herpes simplex virus-1 strain 17+ Bertke et al., 2013 N/A Zika virus, strain Paraiba 2015 (Brazil, 2015) Magnani et al., 2018 N/A SARS-CoV-2 (strain 2019 n-CoV/USA_WA1/2020) CDC/BEI Resources NR52281 Biological Samples Human corneas Mid-America Transplant Institute N/A Chemicals, Peptides, and Recombinant Proteins Recombinant Human IL-29/IFN Lambda 1, HCE PBL assay science Cat# 11725-1 Recombinant Mouse IL-28B/IFN Lambda 3 PBL assay science Cat# 12820-1 Critical Commercial Assays RNeasy Mini Kit QIAGEN Cat# 74104 TaqMan RNA-to-Ct 1-Step Kit ThermoFisher Cat# 4392653 Experimental Models: Cell Lines Vero CCL81 cells ATCC CCL81; RRID: CVCL_0059 Vero E6 cells ATCC CRL-1586; RRID: CVCL_0574 Experimental Models: Organisms/Strains Mouse: Ifnar1 / Jackson Laboratory Cat# JAX:032048, RRID: IMSR_JAX:032048 Mouse: AG129 Marshall BioResources AG129 Mouse: Ifnlr1 / Baldridge et al., 2017 N/A Oligonucleotides SARS-CoV-2 N F: 50 -ATGCTGCAATCGTGCTACAA-3 Hassan et al., 2020 N/A SARS-CoV-2 N R: 50 -GACTGCCGCCTCTGCTC-30 Hassan et al., 2020 N/A SARS-CoV-2 N Probe: 50 -/56-FAM/TCAAGGAAC/ZEN/ AACATTGCCAA/3IABkFQ/-30 Hassan et al., 2020 N/A RNAscope Probe- V-ZIKV Advanced Cell Diagnostics Cat# 467771 RNAscope Probe- Hs-IFNLR1 Advanced Cell Diagnostics Cat# 494771 RNAscope Probe- Hs-IFNL1 Advanced Cell Diagnostics Cat# 412341 IFNL1 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.56a.21113836.g IFNL2 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.56a.38564463 IFNLR1 PrimeTime qPCR Assay Integrated DNA Technologies Hs00417120_m1 IFIT1 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.56a.20769090.g IFIT2 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.58.1099131 IRF7 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.58.24613215.g IFI27 PrimeTime qPCR Assay ThermoFisher Hs01086373_g1 GAPDH PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.39a.22214936 Ifit1 PrimeTime qPCR Assay Integrated DNA Technologies Mm.PT.58.32674307 Ifit2 PrimeTime qPCR Assay Integrated DNA Technologies Mm.PT.58.45910930.g Ifitm3 PrimeTime qPCR Assay Integrated DNA Technologies Mm.PT.56a.29591636 Irf7 PrimeTime qPCR Assay ThermoFisher Mm00516793 (Continued on next page) e1 Cell Reports 33, 108339, November 3, 2020
Recombinant:Article Title: HSV-1 and Zika Virus but Not SARS-CoV-2 Replicate in the Human Cornea and Are Restricted by Corneal Type III Interferon
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human interferon lambda receptor 1 neutralizing mAb PBL assay science Cat# 21885-1 Unable to locate RRID Mouse IgG1 kappa isotype control mAb Sigma-Aldrich Cat# M7894, RRID:AB_1163632 Bacterial and Virus Strains Herpes simplex virus-1 strain 17+ Bertke et al., 2013 N/A Zika virus, strain Paraiba 2015 (Brazil, 2015) Magnani et al., 2018 N/A SARS-CoV-2 (strain 2019 n-CoV/USA_WA1/2020) CDC/BEI Resources NR52281 Biological Samples Human corneas Mid-America Transplant Institute N/A Chemicals, Peptides, and Recombinant Proteins Recombinant Human IL-29/IFN Lambda 1, HCE PBL assay science Cat# 11725-1 Recombinant Mouse IL-28B/IFN Lambda 3 PBL assay science Cat# 12820-1 Critical Commercial Assays RNeasy Mini Kit QIAGEN Cat# 74104 TaqMan RNA-to-Ct 1-Step Kit ThermoFisher Cat# 4392653 Experimental Models: Cell Lines Vero CCL81 cells ATCC CCL81; RRID: CVCL_0059 Vero E6 cells ATCC CRL-1586; RRID: CVCL_0574 Experimental Models: Organisms/Strains Mouse: Ifnar1 / Jackson Laboratory Cat# JAX:032048, RRID: IMSR_JAX:032048 Mouse: AG129 Marshall BioResources AG129 Mouse: Ifnlr1 / Baldridge et al., 2017 N/A Oligonucleotides SARS-CoV-2 N F: 50 -ATGCTGCAATCGTGCTACAA-3 Hassan et al., 2020 N/A SARS-CoV-2 N R: 50 -GACTGCCGCCTCTGCTC-30 Hassan et al., 2020 N/A SARS-CoV-2 N Probe: 50 -/56-FAM/TCAAGGAAC/ZEN/ AACATTGCCAA/3IABkFQ/-30 Hassan et al., 2020 N/A RNAscope Probe- V-ZIKV Advanced Cell Diagnostics Cat# 467771 RNAscope Probe- Hs-IFNLR1 Advanced Cell Diagnostics Cat# 494771 RNAscope Probe- Hs-IFNL1 Advanced Cell Diagnostics Cat# 412341 IFNL1 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.56a.21113836.g IFNL2 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.56a.38564463 IFNLR1 PrimeTime qPCR Assay Integrated DNA Technologies Hs00417120_m1 IFIT1 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.56a.20769090.g IFIT2 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.58.1099131 IRF7 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.58.24613215.g IFI27 PrimeTime qPCR Assay ThermoFisher Hs01086373_g1 GAPDH PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.39a.22214936 Ifit1 PrimeTime qPCR Assay Integrated DNA Technologies Mm.PT.58.32674307 Ifit2 PrimeTime qPCR Assay Integrated DNA Technologies Mm.PT.58.45910930.g Ifitm3 PrimeTime qPCR Assay Integrated DNA Technologies Mm.PT.56a.29591636 Irf7 PrimeTime qPCR Assay ThermoFisher Mm00516793 (Continued on next page) e1 Cell Reports 33, 108339, November 3, 2020
RNAscope:Article Title: HSV-1 and Zika Virus but Not SARS-CoV-2 Replicate in the Human Cornea and Are Restricted by Corneal Type III Interferon
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human interferon lambda receptor 1 neutralizing mAb PBL assay science Cat# 21885-1 Unable to locate RRID Mouse IgG1 kappa isotype control mAb Sigma-Aldrich Cat# M7894, RRID:AB_1163632 Bacterial and Virus Strains Herpes simplex virus-1 strain 17+ Bertke et al., 2013 N/A Zika virus, strain Paraiba 2015 (Brazil, 2015) Magnani et al., 2018 N/A SARS-CoV-2 (strain 2019 n-CoV/USA_WA1/2020) CDC/BEI Resources NR52281 Biological Samples Human corneas Mid-America Transplant Institute N/A Chemicals, Peptides, and Recombinant Proteins Recombinant Human IL-29/IFN Lambda 1, HCE PBL assay science Cat# 11725-1 Recombinant Mouse IL-28B/IFN Lambda 3 PBL assay science Cat# 12820-1 Critical Commercial Assays RNeasy Mini Kit QIAGEN Cat# 74104 TaqMan RNA-to-Ct 1-Step Kit ThermoFisher Cat# 4392653 Experimental Models: Cell Lines Vero CCL81 cells ATCC CCL81; RRID: CVCL_0059 Vero E6 cells ATCC CRL-1586; RRID: CVCL_0574 Experimental Models: Organisms/Strains Mouse: Ifnar1 / Jackson Laboratory Cat# JAX:032048, RRID: IMSR_JAX:032048 Mouse: AG129 Marshall BioResources AG129 Mouse: Ifnlr1 / Baldridge et al., 2017 N/A Oligonucleotides SARS-CoV-2 N F: 50 -ATGCTGCAATCGTGCTACAA-3 Hassan et al., 2020 N/A SARS-CoV-2 N R: 50 -GACTGCCGCCTCTGCTC-30 Hassan et al., 2020 N/A SARS-CoV-2 N Probe: 50 -/56-FAM/TCAAGGAAC/ZEN/ AACATTGCCAA/3IABkFQ/-30 Hassan et al., 2020 N/A RNAscope Probe- V-ZIKV Advanced Cell Diagnostics Cat# 467771 RNAscope Probe- Hs-IFNLR1 Advanced Cell Diagnostics Cat# 494771 RNAscope Probe- Hs-IFNL1 Advanced Cell Diagnostics Cat# 412341 IFNL1 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.56a.21113836.g IFNL2 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.56a.38564463 IFNLR1 PrimeTime qPCR Assay Integrated DNA Technologies Hs00417120_m1 IFIT1 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.56a.20769090.g IFIT2 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.58.1099131 IRF7 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.58.24613215.g IFI27 PrimeTime qPCR Assay ThermoFisher Hs01086373_g1 GAPDH PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.39a.22214936 Ifit1 PrimeTime qPCR Assay Integrated DNA Technologies Mm.PT.58.32674307 Ifit2 PrimeTime qPCR Assay Integrated DNA Technologies Mm.PT.58.45910930.g Ifitm3 PrimeTime qPCR Assay Integrated DNA Technologies Mm.PT.56a.29591636 Irf7 PrimeTime qPCR Assay ThermoFisher Mm00516793 (Continued on next page) e1 Cell Reports 33, 108339, November 3, 2020
Real-time Polymerase Chain Reaction:Article Title: HSV-1 and Zika Virus but Not SARS-CoV-2 Replicate in the Human Cornea and Are Restricted by Corneal Type III Interferon
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human interferon lambda receptor 1 neutralizing mAb PBL assay science Cat# 21885-1 Unable to locate RRID Mouse IgG1 kappa isotype control mAb Sigma-Aldrich Cat# M7894, RRID:AB_1163632 Bacterial and Virus Strains Herpes simplex virus-1 strain 17+ Bertke et al., 2013 N/A Zika virus, strain Paraiba 2015 (Brazil, 2015) Magnani et al., 2018 N/A SARS-CoV-2 (strain 2019 n-CoV/USA_WA1/2020) CDC/BEI Resources NR52281 Biological Samples Human corneas Mid-America Transplant Institute N/A Chemicals, Peptides, and Recombinant Proteins Recombinant Human IL-29/IFN Lambda 1, HCE PBL assay science Cat# 11725-1 Recombinant Mouse IL-28B/IFN Lambda 3 PBL assay science Cat# 12820-1 Critical Commercial Assays RNeasy Mini Kit QIAGEN Cat# 74104 TaqMan RNA-to-Ct 1-Step Kit ThermoFisher Cat# 4392653 Experimental Models: Cell Lines Vero CCL81 cells ATCC CCL81; RRID: CVCL_0059 Vero E6 cells ATCC CRL-1586; RRID: CVCL_0574 Experimental Models: Organisms/Strains Mouse: Ifnar1 / Jackson Laboratory Cat# JAX:032048, RRID: IMSR_JAX:032048 Mouse: AG129 Marshall BioResources AG129 Mouse: Ifnlr1 / Baldridge et al., 2017 N/A Oligonucleotides SARS-CoV-2 N F: 50 -ATGCTGCAATCGTGCTACAA-3 Hassan et al., 2020 N/A SARS-CoV-2 N R: 50 -GACTGCCGCCTCTGCTC-30 Hassan et al., 2020 N/A SARS-CoV-2 N Probe: 50 -/56-FAM/TCAAGGAAC/ZEN/ AACATTGCCAA/3IABkFQ/-30 Hassan et al., 2020 N/A RNAscope Probe- V-ZIKV Advanced Cell Diagnostics Cat# 467771 RNAscope Probe- Hs-IFNLR1 Advanced Cell Diagnostics Cat# 494771 RNAscope Probe- Hs-IFNL1 Advanced Cell Diagnostics Cat# 412341 IFNL1 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.56a.21113836.g IFNL2 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.56a.38564463 IFNLR1 PrimeTime qPCR Assay Integrated DNA Technologies Hs00417120_m1 IFIT1 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.56a.20769090.g IFIT2 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.58.1099131 IRF7 PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.58.24613215.g IFI27 PrimeTime qPCR Assay ThermoFisher Hs01086373_g1 GAPDH PrimeTime qPCR Assay Integrated DNA Technologies Hs.PT.39a.22214936 Ifit1 PrimeTime qPCR Assay Integrated DNA Technologies Mm.PT.58.32674307 Ifit2 PrimeTime qPCR Assay Integrated DNA Technologies Mm.PT.58.45910930.g Ifitm3 PrimeTime qPCR Assay Integrated DNA Technologies Mm.PT.56a.29591636 Irf7 PrimeTime qPCR Assay ThermoFisher Mm00516793 (Continued on next page) e1 Cell Reports 33, 108339, November 3, 2020
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